The cell is the smallest unit of life. Cells have been cultured outside of animals' bodies and used in various biological
experiments and diagnostics, and production of protein and other valuable biomolecules. Cells can be also a promising candidate
of a part of biosensing devices that will detect complex and combination of biomolecules at high sensitivity that could not
be realized with any human-made devices.
However, since single cells easily die, degrade, and require strict physicochemical conditions to our knowledge in animal cell culture, utilization of cells in the engineering contexts needs more breakthrough in handling cells that is possible in methods of engineering. Not only knowledge in behavior and mechanism of cells, but also various engineering viewpoints and approaches needs to be acquired to develop "easy-to-use" cell devices. These engineering approaches include establishing the system to maintain the cell health and the physicochemical conditions (surface properties, media, atmosphere, flow, concentration gradient), in appropriate ranges, building the system to maintain cells in a simple, biologically sterile and reproducible methods, and the system to obtain signals and information from the cells inside.
However, since single cells easily die, degrade, and require strict physicochemical conditions to our knowledge in animal cell culture, utilization of cells in the engineering contexts needs more breakthrough in handling cells that is possible in methods of engineering. Not only knowledge in behavior and mechanism of cells, but also various engineering viewpoints and approaches needs to be acquired to develop "easy-to-use" cell devices. These engineering approaches include establishing the system to maintain the cell health and the physicochemical conditions (surface properties, media, atmosphere, flow, concentration gradient), in appropriate ranges, building the system to maintain cells in a simple, biologically sterile and reproducible methods, and the system to obtain signals and information from the cells inside.
Students will study the topics that will be fundamental to utilize cells in a engineer-friendly ways. These topics will be
selected mainly from the fields of biomaterials, animal cell culture, and microfluidics.
Students will study the method of English literature search and how to read journal articles through preparation of mini-reports and the final presentation.
Students will study the method of English literature search and how to read journal articles through preparation of mini-reports and the final presentation.
- Students should understand the physicochemical principles and conditions that is relevant and/or can be defined in the cell environment.
- Students should understand the methods of cellular assays in biology and other life sciences．
- Students should understand the methods to engineer environment/atmosphere suitable to cell cultivation as "a device".
| Class schedule | HW assignments (Including preparation and review of the class.) | Amount of Time Required | |
|---|---|---|---|
| 1． | Overview of cellular device engineering ○Goals, three technological fields. ○Difference from life sciences. ○Methods for English literature search. Basics in cell culture ○History ○Categories in cell culture ○Parameters describing proliferation |
Handout No.1 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 2． | Cell culture techniques ○Areas and equipment ○Tools and cell culture vessels. ○Steps ○Media and reagents |
Handout No.2 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 3． | Evaluation of cells ○Microscopy for morphology ○Microscopy for flouorescence detection. ○Assessment of cell viability |
Handout No.3 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 4． | 細胞培養の物理化学的条件 ○培地組成と代謝 ○pHと酸素濃度 ○浸透圧 ○壁せん断力 |
Handout No.4 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 5． | Cell isolation, coculture, 3D culture ○Methods for cell sorting ○Spheroids, organoids ○Methods for coculture and 3D culture |
Handout No.5 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 6． | Forces on microfluids ○Characteristics of microfluids ○Nondimensional Navier-Stokes Eq. Laminar flow solution and pressure loss. ○Advection-diffusion ○Surface tension and Laplace pressure |
Handout No.6 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 7． | Microfluidic elements ○Using capillary forces. ○Using electro- or surface chemistry ○Using strain and deformation |
Handout No.7 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 8． | ※Mini-reports submission due (1) Materials for microfluidic devices ○Biocompatible alloys ○Polymers ○Hydrogels |
Handout No.8 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 9． | Miromachining ○Photolithography ○Bonding, surface treatment ○Rapid prototyping (3D printing, soft lith.) |
Handout No.9 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 10． | Biomacromolecules ○Extracellular matrices ○Three S's of tissue engineering ○Types of scaffolds ○Coating, forming of scaffolds ○Signaling molecules |
Handout No.10 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 11． | Biosensing and bioassays ○Enzyme-based biosensors ○Antibody-based biosensors ○Cell viability assays ※ Final presentation assignment |
Handout No.11 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 12． | Biomolecule separation and detection ○Separation based on affinity ○Electrophoresis ○PCR and application ○Physical dection methods (QCM etc) |
Handout No.12 | 60minutes |
| Mini-report preparation | 60minutes | ||
| 13． | Future of microfluidic systems ○Traditional methods work better? ○Simplicity in microfludiic systems ○Collaboration of science and technology Final presentation (1) |
Reading a given review paper | 150minutes |
| Literature search and reading | 300minutes | ||
| 14． | Mini-reports submission due (2) Final Presentation (2) |
Literature search and reading | 320minutes |
| Presentation | 400minutes | ||
| Total. | - | - | 2610minutes |
| Mini-Reports | Presentation | Total. | |
|---|---|---|---|
| 1. | 20% | 5% | 25% |
| 2. | 20% | 15% | 35% |
| 3. | 20% | 20% | 40% |
| Total. | 60% | 40% | - |
This course will give a pass mark (60 points) to students who have successfully understood cell culture methods and cell analysis
methods and have selected, understood
, and presented a paper that describes a engineering-oriented usage of cells.
, and presented a paper that describes a engineering-oriented usage of cells.
No textbook is required. Read the handouts on Scomb beforehand.
Reference books:
岩田博夫他「化学マスター講座 バイオマテリアル」丸善出版
細胞培養に関する成本（例：許南浩編「細胞培養なるほどQ&A」羊土社）
草壁克己・外輪健一郎「マイクロリアクタ入門」米田出版
堀池靖浩・宮原裕二「高分子先端材料One Point バイオチップとバイオセンサー」共立出版
Reference books:
岩田博夫他「化学マスター講座 バイオマテリアル」丸善出版
細胞培養に関する成本（例：許南浩編「細胞培養なるほどQ&A」羊土社）
草壁克己・外輪健一郎「マイクロリアクタ入門」米田出版
堀池靖浩・宮原裕二「高分子先端材料One Point バイオチップとバイオセンサー」共立出版
- Office hours: Wednesday lunch hours (Toyosu) (appointment required)
There will be a time open for discussion after each class (~10 min).
- Course that cultivates a basic problem-solving skills
| Work experience | Work experience and relevance to the course content if applicatable |
|---|---|
| Applicatable | Experience in research in biomicrofluidic field, and working at a startup company developing microfluidic embryo culture systems. |
- 3.GOOD HEALTH AND WELL-BEING
- 9.INDUSTRY, INNOVATION AND INFRASTRUCTURE
Last modified : Sat Jun 20 04:18:20 JST 2020