The purpose of this course is to acquire basic knowledge and skills in basic experiments before attending Experiment of Bioscience
A and B that will be conducted from the second half of the second degree.
The aim of this class is to learn basic biological experimental knowledge and techniques.
- The aim of this class is to learn basic biological experimental knowledge and techniques.
- Enables correctly handle general-purpose devices such as electronic balances and centrifuges
- Use of micropipette enables handling of trace amounts of reagents, etc.
- Enables usage of software and websites required for research correctly
- Discuss the results of your experiments and summarize them in a report
| Class schedule | HW assignments (Including preparation and review of the class.) | Amount of Time Required | |
|---|---|---|---|
| 1. | Guidance: Grouping, explanation of implementation method, explanation of evaluation method. Theme 1, How to write a report: Learn what a report is, what you need to create a report, and how to write a report. |
Read the guidance text and theme 1 text carefully. | 20minutes |
| Review how to write reports well, and use them to create your reports in subsequent exercises. | 30minutes | ||
| Make out a report | 150minutes | ||
| 2. | Theme 2, How to use pipettes and scales: Learn how to use pipettes and balances used in all experiments, learn how to use them and learn how to handle them. | Read the theme 2 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 3. | Theme 3, Measurement of water hardness: Measure the hardness of commercially available drinking water with different hardness. | Read the theme 3 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 4. | Theme 4, Neutralization titration: By adding NaOH solution to acetic acid and phosphoric acid while measuring the pH and drawing a titration curve, Learn about 1) pH meter operation method (including calibration) and 2) action of buffer solution. | Read the theme 4 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 5. | Theme 5, Thin-layer chromatography: Learn how to extract using a separating funnel and qualitative tests using thin-layer chromatography. | Read the theme 5 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 6. | Oral examination, writing and practical examinations on themes 1 to 5. | Review the experiments and lectures so far, and prepare for oral examinations, writing and practical examinations on themes 1-5. | 200minutes |
| 7. | Theme 6, Total phosphorus and total nitrogen determination: Learn the calibration curve method for quantifying nitric acid concentration using ultraviolet absorbance and nitric acid concentration using visible absorbance, by oxidatively decomposing a sample. | Read the theme 6 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 8. | Theme 7, Sugar identification: Learn how to identify and simplify quantification of starch and reducing sugar of raw potato, boiled potato, and amylase digested potato. We consider saccharides, reducing sugars, DNS reaction and amylase enzyme reaction. | Read the theme 7 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 9. | Theme 8, Amylase activity measurement: | Read the theme 8 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 10. | Theme 9, Protein quantification: Learn how to quantify proteins using the Bradford method. Create a calibration curve and use Excel to verify its accuracy. | Read the theme 9 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 11. | Oral examination, writing and practical examinations on themes 6 to 9. | Review the experiments and lectures so far, and prepare for oral examinations, writing and practical examinations on themes 6 to 9. | 200minutes |
| 12. | Theme 10, How to use software 1: Learn how to use software such as ChemDraw and SciFInder. | Read the theme 10 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 13. | Theme 11, How to use the software 2: Learn how to search documents by web browsing. In particular, learn how to quickly search for a target document by using multiple paper databases such as PubMed. | Read the theme 11 text carefully. | 50minutes |
| Make out a report | 150minutes | ||
| 14. | Oral examination, writing and practical examinations on themes 10 and 11. | Review the experiments and lectures so far, and prepare for oral examinations, writing and practical examinations on themes 10 and 11. | 200minutes |
| Total. | - | - | 2800minutes |
| Reports | Oral examination, writing and practical examinations | Total. | |
|---|---|---|---|
| 1. | 10% | 10% | 20% |
| 2. | 10% | 10% | 20% |
| 3. | 10% | 10% | 20% |
| 4. | 10% | 10% | 20% |
| 5. | 20% | 20% | |
| Total. | 60% | 40% | - |
The score of the report for each experimental theme, the oral examination, and the scores of the written and practical examinations
are totaled and evaluated.
Please attend each time at the start of the experiment.
Although there will be no attendance points, the experiment will be started halfway and it is impossible to catch up with others, you will be absent if you are late for no reason.
Other details will be explained at the time of guidance.
Please attend each time at the start of the experiment.
Although there will be no attendance points, the experiment will be started halfway and it is impossible to catch up with others, you will be absent if you are late for no reason.
Other details will be explained at the time of guidance.
- Questions and consultations regarding the experiment should be directed to the second grader's classroom teacher. Make an appointment before you come.
- Since each faculty member in the life science course has each course, please ask questions about each content to each member after making an appointment.
- Course that cultivates a basic problem-solving skills
- Course that cultivates an ability for utilizing knowledge
| Work experience | Work experience and relevance to the course content if applicatable |
|---|---|
| N/A | 該当しない |
Last modified : Sat Mar 21 11:46:42 JST 2020