This short laboratory course aims to provide understanding for foundation and basic techniques for the procedure of molecular
biology for the students who are taking teacher training course. This course is held at summer school.
Through this short laboratory course you might understand foundation and basic techniques of molecular biology.
- Understanding for foundation and basic techniques of molecular biology.
- Understanding how to quantitative analysis biological phenomena.
- Understanding a way of purification and detection of biological molecules.
| Class schedule | HW assignments (Including preparation and review of the class.) | Amount of Time Required | |
|---|---|---|---|
| 1. | Basic training of molecular biology | You learn four big biomolecules in the cell. | 200minutes |
| 2. | Extraction and purification of genome DNA from the cell | You learn DNA. | 200minutes |
| 3. | PCR for specific region of genome DNA and detection | You learn PCR for specific region of genome DNA and agarose electrophoresis | 200minutes |
| 4. | Cultivations of bacterial cells of which harvest desired protein | Stduy about vector consruction and transformation | 200minutes |
| 5. | Extruction and purification of the protein from the bacterial cell | Stduy about ball mil, centrifugaion, affinity chroatography and gel filtration | 200minutes |
| 6. | Detection of fluorescent proteins | Study about fluorescent protein, foundations and applications | 200minutes |
| 7. | Kinetic study of the purified protein (enzyme) 1 | Stduy about enzume kinetics including Michaelis-Menten mechnism, visible absorption spectrometry. | 200minutes |
| 8. | Kinetic study of the purified protein (enzyme) 2 | Stduy about inhibition of enzymes and its mechanistic analysis | 200minutes |
| 9. | Study on the data bases and search engine for the protein 1 | Go thorugh BRENDA, find BioH and the chemical reaction catalyzed by BioH | 200minutes |
| 10. | Study on the data bases and search engine for the protein 2 | Go thorugh EKGG, how BioH perticipate in biotion symthesis? | 200minutes |
| 11. | Study on the data bases and search engine for the protein 3 | Go thorugh EKGG, look for the enzyme of which genetically resemble to BioH | 200minutes |
| 12. | Study on the data bases and search engine for the protein 4 | Is there any enzyme having concensus catalytic traial as BioH? | 200minutes |
| 13. | Study on the data bases and search engine for the protein 5 | Discuss sbout the reason why enzymes have apropriate pH the works. | 200minutes |
| 14. | Study on the data bases and search engine for the protein 5 | Go thorugh PRENDA, EKGG and PDB. Labeling interested protein with FP is useful. However, does FP affect enzymatic activity? | 200minutes |
| Total. | - | - | 2800minutes |
| report paper | Total. | |
|---|---|---|
| 1. | 30% | 30% |
| 2. | 30% | 30% |
| 3. | 40% | 40% |
| Total. | 100% | - |
The credit requires over 60% of total score on the report paper of which include the topics of "goals and the objectives".
Text：You are provided a method on the first time of this course.
Reference book：Biochemistry: The Molecular Basis of Life Sixth Edition in Japanese
Trudy McKee、James R. McKee Atsushi Ichikawa supervisor, Shinichi Fukuoka chief translator （Kagakudojin）
Reference book：Biochemistry: The Molecular Basis of Life Sixth Edition in Japanese
Trudy McKee、James R. McKee Atsushi Ichikawa supervisor, Shinichi Fukuoka chief translator （Kagakudojin）
It is important to learn basic knowledge of organic- and physical-chemistry, specifically organic-biochemistry.
- Lunch time on every Monday is available for me.
- Hamasaki, Monday to Friday 10：00-18：00 You need an appointment by mail, hamie@sic.shibaura-it.ac.jp
- Course that cultivates an ability for utilizing knowledge
- Course that cultivates a basic problem-solving skills
| Work experience | Work experience and relevance to the course content if applicable |
|---|---|
| N/A | 該当しない |
Last modified : Sun Mar 21 15:34:25 JST 2021