Course title
N0040601,N0040602,N0040603,N00406042
Molecular Biology

 hirota yoshihisa
Course description
Molecular biology is the study of understanding and explaining biological phenomena at the molecular level. Molecular biology is a branch of biology that has developed on the basis of microbiology, biochemistry, and genetics. Based on this molecular (cellular) biology, biotechnology (technology to apply living organisms, bioengineering) such as biomacromolecular engineering (genetic engineering, protein engineering, enzyme engineering, glycoscience, lipid engineering) has been rapidly developed, and has greatly contributed to the development of industry, the environment, medicine and pharmaceuticals (pharmacology, etc.), agriculture and food. The goal of this lecture is to enable students to understand and explain life at the molecular level.
Purpose of class
Molecular biology is the basis of biotechnology (technology that applies living organisms, biomacromolecular engineering, etc.) and contributes greatly to the development of industry, the environment, medicine and pharmaceuticals (pharmacology, etc.), agriculture and food. The purpose of this course is to provide students with a broad understanding of the fundamentals of molecular biology (discovery, biomolecules, blueprints, replication, transcription, translation, expression, etc.), which is based on microbiology and biochemistry, as well as the fundamentals of genetic engineering, which is a direct engineering application of molecular (cell) biology. In addition, students will learn the basics of genetic engineering, which is a direct engineering application of molecular (cell) biology.
Goals and objectives
  1. Understand and explain the roles and relationships of DNA, RNA, and proteins in the body.
  2. Explain how DNA is replicated, how RNA is transcribed from DNA, and how RNA is translated into protein.
  3. Explain the relationship between DNA mutation and evolution with specific examples.
  4. Explain to experimental methods and procedures for gene cloning and protein expression.
Language
Japanese(English accepted)
Class schedule

 Class schedule HW assignments (Including preparation and review of the class.) Amount of Time Required
1. Cells and Genomes Prepare and review the textbook chapter 1 and distribution print 150minutes
Review of the small test 30minutes
2. Information polymers (1) Nucleic acids Prepare and review the textbook chapter 2 and distribution print 150minutes
Review of the small test 30minutes
3. Information polymers (2) Proteins Prepare and review the textbook chapter 2 and distribution print 150minutes
Review of the small test 30minutes
4. Eukaryotic genomes Prepare and review the textbook chapter 3 and distribution print 150minutes
Review of the small test 30minutes
5. Prokaryotic genomes Prepare and review the textbook chapter 32 and distribution print 150minutes
Review of the small test 30minutes
6. DNA replication Prepare and review the textbook chapter 4 and distribution print 150minutes
Review of the small test 30minutes
7. Midterm exam and commentary Prepare and review the textbook chapter 1~4 and distribution print 360minutes
8. Transcription and transcriptional regulation Prepare and review the textbook chapter 5 and distribution print 150minutes
Review of the small test 30minutes
9. Post-transcriptional processing Prepare and review the textbook chapter 5 and distribution print 150minutes
Review of the small test 30minutes
10. Translation Prepare and review the textbook chapter 6 and distribution print 150minutes
Review of the small test 30minutes
11. DNA mutation and evolution Prepare and review the textbook chapter 7 and distribution print 150minutes
Review of the small test 30minutes
12. Basics of genetic engineering (1): cloning, restriction enzymes, PCR Prepare and review the textbook chapter 8 and distribution print 150minutes
Review of the small test 30minutes
13. Basics of genetic engineering (2): DNA analysis methods, expression of recombinant proteins Prepare and review the textbook chapter 8 and distribution print 150minutes
Review of the small test 30minutes
14. Final exam and commentary Prepare and review the textbook chapter 5~8 and distribution print 360minutes
Total. - - 2880minutes
Relationship between 'Goals and Objectives' and 'Course Outcomes'

 Midterm exam Final exam Small test Total.
1. 10% 10% 5% 25%
2. 10% 10% 5% 25%
3. 10% 10% 5% 25%
4. 10% 10% 5% 25%
Total. 40% 40% 20% -
Evaluation method and criteria
Evaluate at a total score of 100 points including 40 midterm exams, 40 final exams, and 20 small tests, and pass at least 60%. 60% of the total point is the level that can understand the contents of textbooks and lecture distribution prints and explain the contents to other people.
Textbooks and reference materials
In the class, PPT materials will be distributed and several textbooks will be extensively explained. Students are encouraged to select a book that suits their level and interest.
Textbook: (1) Molecular Biology (Kodansha, by Masato Ikegami and Mitsuru Ebihara) (for beginners)
　　　　 (2) Bruce Alberts et al. "Essential Cell Biology", 4th edition [Nanjiang-do], chapters 1-10. Chapters 1-10. (For intermediate level)
　　　　 (3) Keiko Nakamura and Kenichi Matsubara (translators), THE CELL: Molecular Biology of the Cell, 6th edition, Newton Press. (For advanced students)
Reference books: (1) Akihiko Kondo and Seiji Shibasaki, Genetic Engineering (Basic Biology Text Series 10), Kagaku Dojin, Tokyo.
　　　　 (2)Keiko Nakamura, Molecular Biology of the Watson Gene, 7th edition, Tokyo Denki University Press.
Prerequisites
None
Office hours and How to contact professors for questions
  • Tuesday and Wednesday lunch break, 1 hour after class
  • When you visit, please contact (e-mail etc) in advance
Regionally-oriented
Non-regionally-oriented course
Development of social and professional independence
  • Course that cultivates an ability for utilizing knowledge
  • Course that cultivates a basic self-management skills
  • Course that cultivates a basic problem-solving skills
  • Course that cultivates a basic interpersonal skills
Active-learning course
More than one class is interactive
Course by professor with work experience
Work experience Work experience and relevance to the course content if applicable
Applicable Based on my experience in clinical drug development, I will teach the concept of molecular biology as a fundamental technology of life science, the current latest research, and practical applications using molecular biology.
Education related SDGs:the Sustainable Development Goals
  • 1.NO POVERTY
  • 2.ZERO HUNGER
  • 3.GOOD HEALTH AND WELL-BEING
  • 4.QUALITY EDUCATION
  • 14.LIFE BELOW WATER
  • 15.LIFE ON LAND
Last modified : Sun Mar 21 15:10:50 JST 2021