Course title
N0846201,N0846202,N0846203,N08462042
Experiment of Bioscience B

 SUHARA Yoshitomo

FUSE Hiroyuki

AZHAM ZULKHARNAIN
Course description
This course course will be conducted experiments on organic chemistry and microbiology.
Students learn procedures of synthetic methods for chemical compounds to develop understanding of classes of "Organic chemistry I" and "Organic Chemistry II".This class include how to treat reagents, organic solvents, and glass items for organic synthesis as well as purification and determination of chemical structure.
n the experiments on microbiology, students learn basic experimental methods such as culturing and morphological observation of microorganisms, and experience what they have learned in the lectures of specialized subjects to acquire theory as knowledge through experience and build a foundation for further study.
Purpose of class
To master how treat reagents, organic solvents, and glass items for synthesis of organic compounds.
Goals and objectives
  1. Further understanding of organic chemistry through the experiments.
  2. To master procedure of organic synthesis.
  3. To understand how to write a report.
  4. Learn how to handle microorganisms.
  5. To learn the basics of genetic engineering and be able to practice it.
Language
Japanese
Class schedule

 Class schedule HW assignments (Including preparation and review of the class.) Amount of Time Required
1. Guidance Read the textbook carefully and understand the precautions for the experiment 140minutes
2. Synthesis of Indigo After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 140minutes
Write a report to be submitted the next week.
3. Synthesis of a macrocyclic compound After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
4. Synthesis of aspirin and methyl salicylate After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
5. Synthesis of CYALUME After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
6. Microbiology Experiments After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
7. Microbiology Experiments After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
8. Culture and observation of microorganisms. After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
9. Transformation of E. coli After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
10. Plasmid DNA extraction After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
11. Enzyme expression After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
12. Extraction of enzymes After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
13. Confirmation of enzyme expression After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook. 60minutes
Write a report to be submitted the next week. 180minutes
14. Practical and oral examinations Prepare for examination. 240minutes
Total. - - 3160minutes
Relationship between 'Goals and Objectives' and 'Course Outcomes'

 レポート 実験ノート 試験 Total.
1. 10% 5% 15%
2. 10% 5% 15%
3. 15% 5% 20%
4. 15% 10% 25%
5. 15% 10% 25%
Total. 65% 15% 20% -
Evaluation method and criteria
In organic chemistry experiments, students will be evaluated on the basis of their reports, laboratory notebooks, and attitude toward practical work. In microbiology experiments, students will be further evaluated by examinations (oral and practical).
Textbooks and reference materials
Students are required to bring their own printed copy of the laboratory manual (PDF file).
Prerequisites
Students should review Organic Chemistry I and II and Microbiology.
Office hours and How to contact professors for questions
  • Thursdays and Fridays from 1:00 pm to 5:00 pm. Advance notice of visit is preferred.
Regionally-oriented
Non-regionally-oriented course
Development of social and professional independence
  • Course that cultivates an ability for utilizing knowledge
  • Course that cultivates a basic self-management skills
  • Course that cultivates a basic problem-solving skills
Active-learning course
About half of the classes are interactive
Course by professor with work experience
Work experience Work experience and relevance to the course content if applicable
Applicable Using experiences in synthetic organic chemistry research, this class teaches the basic knowledge and operation methods necessary for organic chemistry experiments.
Education related SDGs:the Sustainable Development Goals
  • 6.CLEAN WATER AND SANITATION
  • 9.INDUSTRY, INNOVATION AND INFRASTRUCTURE
Last modified : Mon May 23 04:25:17 JST 2022