Course title
N0846302,N0846303,N08463043
Experiment of Bioscience C

 FUKUI Koji

OSAKABE Naomi

YAJIMA Ichiro

HIROTA Yoshihisa
Course description
In this experiment, students learn techniques and knowledge to apply the experimental techniques and knowledge acquired in Basic Life Science Experiments and Life Science Experiments A and B to experiments using cells and individual animals used in general research.
Purpose of class
The goal of this experiment is to acquire the animal experiment and molecular biology experimental techniques that will be used in the comprehensive research conducted in the fourth year of the course. In animal experiments, students will begin with the basic knowledge (code of ethics and various laws and regulations) necessary for the proper care and keeping of laboratory animals, learn basic techniques (holding, administration, anesthesia, and dissection) using actual rodents, and verify these techniques using biochemical or histological analysis methods. Students will also learn experimental techniques for detecting genes using polymerase chain reaction (PCR) and electrophoresis as molecular biological experimental techniques.
Goals and objectives
  1. Understand the laws and ethical guidelines for conducting animal and genetic experiments, and be able to handle samples such as organs and blood safely and effectively.
  2. Ability to perform basic animal experiments on rodents
  3. Genes from biological samples can be used to obtain valid information
  4. Organs from biological samples can be used to obtain valid information
  5. Be able to discuss experimental results using basic statistical methods based on the data obtained
Relationship between 'Goals and Objectives' and 'Course Outcomes'

 Experimental Report subject Total.
1. 15% 5% 20%
2. 15% 5% 20%
3. 15% 5% 20%
4. 15% 5% 20%
5. 15% 5% 20%
Total. 75% 25% -
Language
Japanese
Class schedule

 Class schedule HW assignments (Including preparation and review of the class.) Amount of Time Required
1. Animal experiment guidance (laws, regulations and ethical guidelines related to animal experiments) Read the syllabus and lab book carefully 30minutes
Perform the assignment 45minutes
2. Dietary Survey Week 1 Report writing and submission 500minutes
3. Dietary Survey Week 2 Report writing and submission 500minutes
4. Biostatistics and its applications Report writing and submission 500minutes
5. Preparation of reagents for experiments Report writing and submission 500minutes
6. Confirmation of physiological changes in rats ingested high-fat diet (1) Organ findings Report writing and submission 500minutes
7. Confirmation of physiological changes in rats ingested high-fat diet (2) Hematological findings Report writing and submission 500minutes
8. Confirmation of physiological changes in rats fed a high-fat diet (3) Liver TBARS Report writing and submission 500minutes
9. Histopathological observation method (1) (using microscope) Report writing and submission 500minutes
10. Histopathological observation method 2 (observation of pathological sections) Report writing and submission 500minutes
11. Guidance on genetic experiments (laws, regulations and ethical guidelines for conducting genetic experiments) Report writing and submission 500minutes
12. Gene extraction and amplification from biological samples Report writing and submission 500minutes
13. Analysis of genetic information obtained from biological samples Report writing and submission 500minutes
14. Report check and review Report writing and submission 500minutes
Total. - - 6575minutes
Evaluation method and criteria
Students are responsible for printing out their own copies of the lab book from the distribution folder.
Feedback on exams, assignments, etc.
ways of feedback specific contents about "Other"
Feedback in the class
Textbooks and reference materials
A report will be submitted for each experiment, which together will be worth 75 points. The contents of the reports will be explained in each experiment. The student will submit an assignment on practical skills, which together will be worth 25 points. The final evaluation will be made by totaling these two scores. The passing score will be 60 points, which is considered to be a general understanding of the course content.
Prerequisites
Review of biochemistry, food nutrition, and molecular biology
Office hours and How to contact professors for questions
  • Questions will be accepted on Mondays and Thursdays (12:30-15:00).
Regionally-oriented
Non-regionally-oriented course
Development of social and professional independence
  • Course that cultivates an ability for utilizing knowledge
Active-learning course
Most classes are interactive
Course by professor with work experience
Work experience Work experience and relevance to the course content if applicable
Applicable Faculty members in charge have experience in research and development work at pharmaceutical and food manufacturers.
Education related SDGs:the Sustainable Development Goals
  • 3.GOOD HEALTH AND WELL-BEING
Last modified : Fri Jun 28 17:11:14 JST 2024