Experiment of Bioscience B

Course title

N0846201

,N0846204

	HIROTA Yoshihisa
	KAWASHIMA Hiroto
	SUHARA Yoshitomo
	AZHAM ZULKHARNAIN

Course description

This course course will be conducted experiments on organic chemistry, microbiology and biochemistry.
Students learn procedures of synthetic methods for chemical compounds to develop understanding of classes of "Organic chemistry I" and "Organic Chemistry II".This class include how to treat reagents, organic solvents, and glass items for organic synthesis as well as purification and determination of chemical structure.
n the experiments on microbiology, students learn basic experimental methods such as culturing and morphological observation of microorganisms, and experience what they have learned in the lectures of specialized subjects to acquire theory as knowledge through experience and build a foundation for further study.
Biochemistry experiments are conducted through the accurate measurement of vitamins in foods and the calculation of the hardness of water around the world. In this study, students will master biochemistry experiments and analytical techniques other than biological experiments, such as fluorescence measurement and the use of burette.

Purpose of class

Students will deepen their understanding of the reactivity and reaction mechanisms of organic compounds, microorganisms, genetic engineering, and analytical techniques essential to biochemistry through experiments.

Goals and objectives

Further understanding of organic chemistry through the experiments.
To master procedure of organic synthesis.
To understand how to write a report.
Learn how to handle microorganisms.
To learn the basics of genetic engineering and and biochemical analysis be able to practice it.

Relationship between 'Goals and Objectives' and 'Course Outcomes'

	レポート	実験ノート	試験	Total.
1.	10%	5%		15%
2.	10%	5%		15%
3.	15%	5%		20%
4.	15%		10%	25%
5.	15%		10%	25%
Total.	65%	15%	20%	-

Language

Japanese

Class schedule

	Class schedule	HW assignments (Including preparation and review of the class.)	Amount of Time Required
1.	Guidance Synthesis of Indigo	Read the textbook carefully and understand the precautions for the experiment	140minutes
2.	Synthesis of aspirin and methyl salicylate	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	140minutes
2.	Synthesis of aspirin and methyl salicylate	Write a report to be submitted the next week.
3.	Synthesis of a macrocyclic compound	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
3.	Synthesis of a macrocyclic compound	Write a report to be submitted the next week.	180minutes
4.	Synthesis of CYALUME	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
4.	Synthesis of CYALUME	Write a report to be submitted the next week.	180minutes
5.	5th	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
5.	5th	Write a report to be submitted the next week.	180minutes
6.	Microbiology Experiments	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
6.	Microbiology Experiments	Write a report to be submitted the next week.	180minutes
7.	Microbiology Experiments	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
7.	Microbiology Experiments	Write a report to be submitted the next week.	180minutes
8.	Culture and observation of microorganisms.	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
8.	Culture and observation of microorganisms.	Write a report to be submitted the next week.	180minutes
9.	Transformation of E. coli	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
9.	Transformation of E. coli	Write a report to be submitted the next week.	180minutes
10.	Plasmid DNA extraction	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
10.	Plasmid DNA extraction	Write a report to be submitted the next week.	180minutes
11.	Enzyme expression	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
11.	Enzyme expression	Write a report to be submitted the next week.	180minutes
12.	Extraction of enzymes Confirmation of enzyme expression	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
12.	Extraction of enzymes Confirmation of enzyme expression	Write a report to be submitted the next week.	180minutes
13.	Extraction and determination of vitamin B2 from food ingredients	After reading the textbook and understanding the contents, summarize the experimental procedure as a flowchart in the experimental notebook.	60minutes
13.		Write a report to be submitted the next week.	180minutes
14.	Determination of water hardness around the world	Prepare for examination.	60minutes
14.	Determination of water hardness around the world		180minutes
Total.	-	-	3160minutes

Evaluation method and criteria

In organic chemistry experiments, students will be evaluated on the basis of their reports, laboratory notebooks, and attitude toward practical work. In microbiology experiments, students will be further evaluated by examinations (oral and practical).
In the biochemistry experiment, the report (80 points) and the experiment notebook (20 points) will be evaluated based on the student's attitude towards the practical training, and a total of 60 points out of 100 points is required to pass. The 60 points in this experiment is the level of report that can be written after doing the preparation and attending the experiment to the end.

Feedback on exams, assignments, etc.

ways of feedback	specific contents about "Other"
Feedback in the class

Textbooks and reference materials

Students are required to bring their own printed copy of the laboratory manual (PDF file).

Prerequisites

Students should review Organic Chemistry I and II and Microbiology, biochemistry.

Office hours and How to contact professors for questions

Thursdays and Fridays from 1:00 pm to 5:00 pm. Advance notice of visit is preferred.

Regionally-oriented

Non-regionally-oriented course

Development of social and professional independence

Course that cultivates an ability for utilizing knowledge
Course that cultivates a basic self-management skills
Course that cultivates a basic problem-solving skills

Active-learning course

About half of the classes are interactive

Course by professor with work experience

Work experience	Work experience and relevance to the course content if applicable
Applicable	Teaching the concept of biochemistry as a fundamental technology of life science, current state-of-the-art research, and practical applications using biochemistry.

Education related SDGs:the Sustainable Development Goals

3.GOOD HEALTH AND WELL-BEING
4.QUALITY EDUCATION
6.CLEAN WATER AND SANITATION
9.INDUSTRY, INNOVATION AND INFRASTRUCTURE

Last modified : Mon Apr 28 04:03:50 JST 2025