Course title
D05407002
Laboratory Course of Biological Chemistry

 HAMASAKI Keita

HIROI Takashi

LI QINTONG
Course description
This short laboratory course aims to provide understanding for foundation and basic techniques for the procedure of molecular biology for the students who are taking teacher training course. This course is held at summer school.
Purpose of class
Through this short laboratory course you might understand foundation and basic techniques of molecular biology.
Goals and objectives
  1. Understanding for foundation and basic techniques of molecular biology.
  2. Understanding how to quantitative analysis biological phenomena.
  3. Understanding a way of purification and detection of biological molecules.
Language
Japanese
Class schedule
Class schedule HW assignments (Including preparation and review of the class.) Amount of Time Required
Foundations of molecular biology Polynucleotide, polypeptide, polysacchride 200minutes
Chemicals of which construct DNA and RNA and teor rolles Ribose, nucleobase and phosohate 200minutes
Polymerization of mononucreotide to polynucleotide Nucleophilic substitution reaction of mononucleotide triphosphate 200minutes
Chemical reactions catalyzed by nuclease Nucleophilic substitution reaction
Purification of the protein with affinity chromatography and gel filtration Study about affinity chromatography and gel filtration
Replication of specific DNA sequence in genome by PCR Polymerase chain reaction
Electrophoresis of nucleic acids Electrophoresis
Staining of the DNA in the agarose gel Intercalation of the dye to the DNA
The nature of fluorescent protein and fluorescence dye Fluorescence protein and fluorescence dye. How to construct a fusion protein
Syntheisis of the fluorescent proetin labled enzyme, FP-BioH Translation of genetic information
Extruction of the fluorescent proetin labled enzyme, FP-BioH Centrifugaion, Disruption of the cell
Purification of the fluorescent proetin labled enzyme, FP-BioH Affinity chromatography and gel filtration
Affinity chromatography Histidine tag and chelation to cobalt
Gel filtaration, eleimination of small chemicals include salt Size exclurion chromatography, how it works?
Enzyme kinetics Hw can we know the rate of the exzymatic reaction
- - 600minutes
Relationship between 'Goals and Objectives' and 'Course Outcomes'

 Report paper Total.
1. 30% 30%
2. 30% 30%
3. 40% 40%
Total. 100% -
Evaluation method and criteria
The credit requires over 60% of total score on the report paper of which include the topics of "goals and the objectives".
Feedback on exams, assignments, etc.
ways of feedback specific contents about "Other"
Feedback in outside of the class (ScombZ, mail, etc.)
Textbooks and reference materials
Download the protocol from scomb2
Prerequisites
Organic, inorganic analytical and physical chemistry
Office hours and How to contact professors for questions
  • scomb2 message to the instractor
Regionally-oriented
Non-regionally-oriented course
Development of social and professional independence
  • Course that cultivates an ability for utilizing knowledge
Active-learning course
Most classes are interactive
Course by professor with work experience
Work experience Work experience and relevance to the course content if applicable
N/A
Education related SDGs:the Sustainable Development Goals
    Last modified : Sat Sep 09 05:34:53 JST 2023